Ameliorative Effect of an Aqueous Mixture of Cinnamon and Ginger Tea Against Hepatotoxicity Induced in Rats by the Insecticide Diazinon: A Histopathological and Ultrastructural Study

The present study aims to investigate the ameliorative role of an aqueous powders mixture of cinnamon and ginger tea against the insecticide diazinon-induced hepatotoxicity in adult male Sprague-Dawley white rats. The oral LD50 of diazinon “850.12 mg/kg body weight rat” was determined during the current investigation. The light microscope examination of the liver sections of the rats orally-treated with 1/10 LD50 doses of diazinon daily for the last 10 days of the 90 days of the experiment revealed damage of the liver architecture, cytoplasmic vacuolization of the hepatocytes, pyknosis or karyolysis of the liver cells’ nuclei, necrosis, blood congestion, widening of blood sinusoids, hypertrophy of Kupffer cells, infiltration of inflammatory cells. Also, examination of the liver tissue of the same group of rats by using the electron microscope showed presence of cytopathological alterations such as swelling of endoplasmic reticulum, degeneration of the mitochondria and Golgi apparatus. The blood sinusoids were markedly widened and contained residual bodies of the necrotic liver cells. The bile canaliculi exhibited deformation and their microvilli became very few and appeared as ghosts. All these previously-mentioned pathological changes were not noticed in the liver sections of the rats that were orally-given the cinnamon and ginger tea for 90 days, and orallytreated with 1/10 LD50 doses of diazinon daily for the last 10 days of the 90 days of the experiment. Therefore, the current results prove the potent ameliorative activity of the aqueous mixture of cinnamon and ginger tea against hepatotoxicity induced in rats by the insecticide diazinon.